holm-sidak multiple t tests Search Results


tissue specimens of nevus, primary and metastatic melanoma  (Hormel Health Labs)
90
Hormel Health Labs tissue specimens of nevus, primary and metastatic melanoma
Tissue Specimens Of Nevus, Primary And Metastatic Melanoma, supplied by Hormel Health Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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immunoassay kit  (Meso Scale Diagnostics LLC)
90
Meso Scale Diagnostics LLC immunoassay kit
Construction and in vitro evaluation of HER2 CAR T cells. a To create anti-HER2 CAR T cells, the 4D5 anti-HER2 scFv was ligated into a retroviral backbone vector containing a CD3 zeta chain signaling domain and 4-1BB costimulatory domain. Human peripheral blood mononuclear cells (PBMCs) from healthy donors were activated with anti-CD3/anti-CD28 beads on Day 0 and serially transduced with retrovirus containing the CAR construct on days 2 and 3. Beads were removed on day 4 and T cells were allowed to expand until day 9–11, at which point CAR T cells were harvested. Anti-HER2 CAR expression was assessed by Protein L staining and flow cytometry on Day 4. Representative Protein L staining results are shown for mock transduced T cells, CD19 CAR T cells, and HER2 CAR T cells. b HER2 Expression was assessed for the DAOY, D283, and D425 human medulloblastoma cell lines via either qPCR (left) or flow cytometry using the trastuzumab-APC antibody (right). The 928 cell line was used as a positive control for flow cytometry. c In a 24-h Incucyte Zoom assay, tumor cells were co-cultured with CD19 CAR or HER2 CAR transduced T cells at the indicated ratios or with trastuzumab. Percent total live tumor cells was measured compared to 100% at time zero. The percentage of live tumor cells at the 24 h time point was compared for each condition using a one-way ANOVA with Sidak’s correction for multiple hypothesis testing. Statistics are shown for the comparison of HER2 CAR 2.5:1 and CD19 CAR 10:1 for all three lines, as well as the comparison of trastuzumab and HER2 CAR 2.5:1 for D425. Denotion of “n.s” indicates a p value greater than 0.95. d Tumor cells were co-cultured for 24 h with CD19 CAR or HER2 CAR transduced T cells at a 1:1 ratio. IFNγ, IL-2, and TNFα production was measured by a Meso Scale Discovery <t>immunoassay</t> kit, and compared for each condition using multiple T tests with the Holm-Sidak correction
Immunoassay Kit, supplied by Meso Scale Diagnostics LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/holm-sidak+multiple+t+tests/immunoassay+kits/pmc05925833-61-11-9
Average 90 stars, based on 1 article reviews
immunoassay kit - by Bioz Stars, 2026-09
90/100 stars
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digitalmicrograph software  (Gatan Inc)
93
Gatan Inc digitalmicrograph software
Construction and in vitro evaluation of HER2 CAR T cells. a To create anti-HER2 CAR T cells, the 4D5 anti-HER2 scFv was ligated into a retroviral backbone vector containing a CD3 zeta chain signaling domain and 4-1BB costimulatory domain. Human peripheral blood mononuclear cells (PBMCs) from healthy donors were activated with anti-CD3/anti-CD28 beads on Day 0 and serially transduced with retrovirus containing the CAR construct on days 2 and 3. Beads were removed on day 4 and T cells were allowed to expand until day 9–11, at which point CAR T cells were harvested. Anti-HER2 CAR expression was assessed by Protein L staining and flow cytometry on Day 4. Representative Protein L staining results are shown for mock transduced T cells, CD19 CAR T cells, and HER2 CAR T cells. b HER2 Expression was assessed for the DAOY, D283, and D425 human medulloblastoma cell lines via either qPCR (left) or flow cytometry using the trastuzumab-APC antibody (right). The 928 cell line was used as a positive control for flow cytometry. c In a 24-h Incucyte Zoom assay, tumor cells were co-cultured with CD19 CAR or HER2 CAR transduced T cells at the indicated ratios or with trastuzumab. Percent total live tumor cells was measured compared to 100% at time zero. The percentage of live tumor cells at the 24 h time point was compared for each condition using a one-way ANOVA with Sidak’s correction for multiple hypothesis testing. Statistics are shown for the comparison of HER2 CAR 2.5:1 and CD19 CAR 10:1 for all three lines, as well as the comparison of trastuzumab and HER2 CAR 2.5:1 for D425. Denotion of “n.s” indicates a p value greater than 0.95. d Tumor cells were co-cultured for 24 h with CD19 CAR or HER2 CAR transduced T cells at a 1:1 ratio. IFNγ, IL-2, and TNFα production was measured by a Meso Scale Discovery <t>immunoassay</t> kit, and compared for each condition using multiple T tests with the Holm-Sidak correction
Digitalmicrograph Software, supplied by Gatan Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
digitalmicrograph software - by Bioz Stars, 2026-09
93/100 stars
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holm–sidak method  (CH Instruments)
90
CH Instruments holm–sidak method
Construction and in vitro evaluation of HER2 CAR T cells. a To create anti-HER2 CAR T cells, the 4D5 anti-HER2 scFv was ligated into a retroviral backbone vector containing a CD3 zeta chain signaling domain and 4-1BB costimulatory domain. Human peripheral blood mononuclear cells (PBMCs) from healthy donors were activated with anti-CD3/anti-CD28 beads on Day 0 and serially transduced with retrovirus containing the CAR construct on days 2 and 3. Beads were removed on day 4 and T cells were allowed to expand until day 9–11, at which point CAR T cells were harvested. Anti-HER2 CAR expression was assessed by Protein L staining and flow cytometry on Day 4. Representative Protein L staining results are shown for mock transduced T cells, CD19 CAR T cells, and HER2 CAR T cells. b HER2 Expression was assessed for the DAOY, D283, and D425 human medulloblastoma cell lines via either qPCR (left) or flow cytometry using the trastuzumab-APC antibody (right). The 928 cell line was used as a positive control for flow cytometry. c In a 24-h Incucyte Zoom assay, tumor cells were co-cultured with CD19 CAR or HER2 CAR transduced T cells at the indicated ratios or with trastuzumab. Percent total live tumor cells was measured compared to 100% at time zero. The percentage of live tumor cells at the 24 h time point was compared for each condition using a one-way ANOVA with Sidak’s correction for multiple hypothesis testing. Statistics are shown for the comparison of HER2 CAR 2.5:1 and CD19 CAR 10:1 for all three lines, as well as the comparison of trastuzumab and HER2 CAR 2.5:1 for D425. Denotion of “n.s” indicates a p value greater than 0.95. d Tumor cells were co-cultured for 24 h with CD19 CAR or HER2 CAR transduced T cells at a 1:1 ratio. IFNγ, IL-2, and TNFα production was measured by a Meso Scale Discovery <t>immunoassay</t> kit, and compared for each condition using multiple T tests with the Holm-Sidak correction
Holm–Sidak Method, supplied by CH Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/holm-sidak+multiple+t+tests/holm+sidak+method/pmc08379269-132-26-95
Average 90 stars, based on 1 article reviews
holm–sidak method - by Bioz Stars, 2026-09
90/100 stars
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chi-squared test  (CH Instruments)
90
CH Instruments chi-squared test
Construction and in vitro evaluation of HER2 CAR T cells. a To create anti-HER2 CAR T cells, the 4D5 anti-HER2 scFv was ligated into a retroviral backbone vector containing a CD3 zeta chain signaling domain and 4-1BB costimulatory domain. Human peripheral blood mononuclear cells (PBMCs) from healthy donors were activated with anti-CD3/anti-CD28 beads on Day 0 and serially transduced with retrovirus containing the CAR construct on days 2 and 3. Beads were removed on day 4 and T cells were allowed to expand until day 9–11, at which point CAR T cells were harvested. Anti-HER2 CAR expression was assessed by Protein L staining and flow cytometry on Day 4. Representative Protein L staining results are shown for mock transduced T cells, CD19 CAR T cells, and HER2 CAR T cells. b HER2 Expression was assessed for the DAOY, D283, and D425 human medulloblastoma cell lines via either qPCR (left) or flow cytometry using the trastuzumab-APC antibody (right). The 928 cell line was used as a positive control for flow cytometry. c In a 24-h Incucyte Zoom assay, tumor cells were co-cultured with CD19 CAR or HER2 CAR transduced T cells at the indicated ratios or with trastuzumab. Percent total live tumor cells was measured compared to 100% at time zero. The percentage of live tumor cells at the 24 h time point was compared for each condition using a one-way ANOVA with Sidak’s correction for multiple hypothesis testing. Statistics are shown for the comparison of HER2 CAR 2.5:1 and CD19 CAR 10:1 for all three lines, as well as the comparison of trastuzumab and HER2 CAR 2.5:1 for D425. Denotion of “n.s” indicates a p value greater than 0.95. d Tumor cells were co-cultured for 24 h with CD19 CAR or HER2 CAR transduced T cells at a 1:1 ratio. IFNγ, IL-2, and TNFα production was measured by a Meso Scale Discovery <t>immunoassay</t> kit, and compared for each condition using multiple T tests with the Holm-Sidak correction
Chi Squared Test, supplied by CH Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/holm-sidak+multiple+t+tests/chi+square+test/10__1002_slash_jlb__2a1021___564r-122-15-15
Average 90 stars, based on 1 article reviews
chi-squared test - by Bioz Stars, 2026-09
90/100 stars
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sigmastat software  (SYSTAT)
96
SYSTAT sigmastat software
Construction and in vitro evaluation of HER2 CAR T cells. a To create anti-HER2 CAR T cells, the 4D5 anti-HER2 scFv was ligated into a retroviral backbone vector containing a CD3 zeta chain signaling domain and 4-1BB costimulatory domain. Human peripheral blood mononuclear cells (PBMCs) from healthy donors were activated with anti-CD3/anti-CD28 beads on Day 0 and serially transduced with retrovirus containing the CAR construct on days 2 and 3. Beads were removed on day 4 and T cells were allowed to expand until day 9–11, at which point CAR T cells were harvested. Anti-HER2 CAR expression was assessed by Protein L staining and flow cytometry on Day 4. Representative Protein L staining results are shown for mock transduced T cells, CD19 CAR T cells, and HER2 CAR T cells. b HER2 Expression was assessed for the DAOY, D283, and D425 human medulloblastoma cell lines via either qPCR (left) or flow cytometry using the trastuzumab-APC antibody (right). The 928 cell line was used as a positive control for flow cytometry. c In a 24-h Incucyte Zoom assay, tumor cells were co-cultured with CD19 CAR or HER2 CAR transduced T cells at the indicated ratios or with trastuzumab. Percent total live tumor cells was measured compared to 100% at time zero. The percentage of live tumor cells at the 24 h time point was compared for each condition using a one-way ANOVA with Sidak’s correction for multiple hypothesis testing. Statistics are shown for the comparison of HER2 CAR 2.5:1 and CD19 CAR 10:1 for all three lines, as well as the comparison of trastuzumab and HER2 CAR 2.5:1 for D425. Denotion of “n.s” indicates a p value greater than 0.95. d Tumor cells were co-cultured for 24 h with CD19 CAR or HER2 CAR transduced T cells at a 1:1 ratio. IFNγ, IL-2, and TNFα production was measured by a Meso Scale Discovery <t>immunoassay</t> kit, and compared for each condition using multiple T tests with the Holm-Sidak correction
Sigmastat Software, supplied by SYSTAT, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/holm-sidak+multiple+t+tests/SigmaStat+v4%2E0/pmc04910297-105-28-32
Average 96 stars, based on 1 article reviews
sigmastat software - by Bioz Stars, 2026-09
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Image Search Results


Construction and in vitro evaluation of HER2 CAR T cells. a To create anti-HER2 CAR T cells, the 4D5 anti-HER2 scFv was ligated into a retroviral backbone vector containing a CD3 zeta chain signaling domain and 4-1BB costimulatory domain. Human peripheral blood mononuclear cells (PBMCs) from healthy donors were activated with anti-CD3/anti-CD28 beads on Day 0 and serially transduced with retrovirus containing the CAR construct on days 2 and 3. Beads were removed on day 4 and T cells were allowed to expand until day 9–11, at which point CAR T cells were harvested. Anti-HER2 CAR expression was assessed by Protein L staining and flow cytometry on Day 4. Representative Protein L staining results are shown for mock transduced T cells, CD19 CAR T cells, and HER2 CAR T cells. b HER2 Expression was assessed for the DAOY, D283, and D425 human medulloblastoma cell lines via either qPCR (left) or flow cytometry using the trastuzumab-APC antibody (right). The 928 cell line was used as a positive control for flow cytometry. c In a 24-h Incucyte Zoom assay, tumor cells were co-cultured with CD19 CAR or HER2 CAR transduced T cells at the indicated ratios or with trastuzumab. Percent total live tumor cells was measured compared to 100% at time zero. The percentage of live tumor cells at the 24 h time point was compared for each condition using a one-way ANOVA with Sidak’s correction for multiple hypothesis testing. Statistics are shown for the comparison of HER2 CAR 2.5:1 and CD19 CAR 10:1 for all three lines, as well as the comparison of trastuzumab and HER2 CAR 2.5:1 for D425. Denotion of “n.s” indicates a p value greater than 0.95. d Tumor cells were co-cultured for 24 h with CD19 CAR or HER2 CAR transduced T cells at a 1:1 ratio. IFNγ, IL-2, and TNFα production was measured by a Meso Scale Discovery immunoassay kit, and compared for each condition using multiple T tests with the Holm-Sidak correction

Journal: Journal for Immunotherapy of Cancer

Article Title: Durable regression of Medulloblastoma after regional and intravenous delivery of anti-HER2 chimeric antigen receptor T cells

doi: 10.1186/s40425-018-0340-z

Figure Lengend Snippet: Construction and in vitro evaluation of HER2 CAR T cells. a To create anti-HER2 CAR T cells, the 4D5 anti-HER2 scFv was ligated into a retroviral backbone vector containing a CD3 zeta chain signaling domain and 4-1BB costimulatory domain. Human peripheral blood mononuclear cells (PBMCs) from healthy donors were activated with anti-CD3/anti-CD28 beads on Day 0 and serially transduced with retrovirus containing the CAR construct on days 2 and 3. Beads were removed on day 4 and T cells were allowed to expand until day 9–11, at which point CAR T cells were harvested. Anti-HER2 CAR expression was assessed by Protein L staining and flow cytometry on Day 4. Representative Protein L staining results are shown for mock transduced T cells, CD19 CAR T cells, and HER2 CAR T cells. b HER2 Expression was assessed for the DAOY, D283, and D425 human medulloblastoma cell lines via either qPCR (left) or flow cytometry using the trastuzumab-APC antibody (right). The 928 cell line was used as a positive control for flow cytometry. c In a 24-h Incucyte Zoom assay, tumor cells were co-cultured with CD19 CAR or HER2 CAR transduced T cells at the indicated ratios or with trastuzumab. Percent total live tumor cells was measured compared to 100% at time zero. The percentage of live tumor cells at the 24 h time point was compared for each condition using a one-way ANOVA with Sidak’s correction for multiple hypothesis testing. Statistics are shown for the comparison of HER2 CAR 2.5:1 and CD19 CAR 10:1 for all three lines, as well as the comparison of trastuzumab and HER2 CAR 2.5:1 for D425. Denotion of “n.s” indicates a p value greater than 0.95. d Tumor cells were co-cultured for 24 h with CD19 CAR or HER2 CAR transduced T cells at a 1:1 ratio. IFNγ, IL-2, and TNFα production was measured by a Meso Scale Discovery immunoassay kit, and compared for each condition using multiple T tests with the Holm-Sidak correction

Article Snippet: IFNγ, IL-2, and TNFα production was measured by a Meso Scale Discovery immunoassay kit, and compared for each condition using multiple T tests with the Holm-Sidak correction

Techniques: In Vitro, Retroviral, Plasmid Preparation, Transduction, Construct, Expressing, Staining, Flow Cytometry, Positive Control, Cell Culture, Comparison